STANTON GELVIN Edwin Umbarger Distinguished Professor of Biology 765-494-4939 HANS 339 gelvin@purdue.edu Associated website(s): NSF Project: Identification of Plant Genes Involved in Agrobacterium-Mediated Transformation , NSF Project: Bimolecular fluorescence complementation (BMFC) to investigate protein-protein interactions in planta , Motility Group , Publications

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PROFESSIONAL FACULTY RESEARCH

(Plant molecular biology) Mechanism of the transfer to and expression of the Agrobacterium tumefaciens Ti-plasmid in plant cells.

BIO

Our research investigates how a soil bacterium, Agrobacterium tumefaciens , genetically engineers plants. Agrobacterium transfers a piece of bacterial DNA, the T-(transferred) DNA, to wounded plant cells where it makes its way through the cytoplasm to the nucleus. Once in the nucleus, T-DNA integrates into the host genome and expresses genes. Under normal circumstances, these genes cause the disease Crown Gall on plants. However, scientists have learned to manipulate T-DNA, replacing disease genes with genes of benefit to the plant. Many genetically engineered crop plants with desirable traits (disease resistance, herbicide tolerance, and enhanced nutritional value) were generated using Agrobacterium . Unfortunately, many important crop plants, including those important to Indiana farmers (corn, soybeans, and wheat) remain highly recalcitrant to Agrobacterium -mediated genetic transformation.

Our research focuses on understanding the role of plant genes and proteins in this natural genetic engineering process. We have identified plant genes involved in bacterial attachment to plant cells, T-DNA and Virulence transfer to and cytoplasmic trafficking within plants , T-DNA nuclear targeting, and T-DNA integration. Recently, we have been able to manipulate some of these genes to improve Agrobacterium transformation efficiency. We are currently working with agricultural biotechnology companies to improve the genetic engineering of crops, including those important for Indianas economy.

Education

Ph.D., California, San Diego, 1977

Professional Faculty Research

(Plant molecular biology) Mechanism of the transfer to and expression of the Agrobacterium tumefaciens Ti-plasmid in plant cells.

Awards

• Edwin Umbarger Distinguished Professor of Biology (2008)
• Loomis Lecturer, Iowa State University (2006)
• Fellow, American Academy of Microbiology (elected 2006)
• Fellow, American Association for the Advancement of Science (elected 2010)
• Bindley Bioscience Center Fellow (2012)

Grants

Gelvin (PI) 04/01/16-03/31/17 JSPS Long-term fellowship

Gelvin (PI) 04/01/17-04/14/20 USDA Exploring next generation sweetpotato breeding with CRISPR associated proteins

Gelvin (PI) 08/01/17-7/31/20 NSF TRANSFORM-PGR: Manipulating Agrobacterium-mediate transformation and T-DNA integration for plant synthetic biology and genome editing

Gelvin (PI) 08/01/17-7/31/20 (currently on no-cost extension) NSF TRANSFORM-PGR: Manipulating Agrobacterium-mediate transformation and T-DNA integration for plant synthetic biology and genome editing

Gelvin, S.B. (PI) and West, C. (co-PI) 02/15/2019-01/31/2021 (currently on no-cost extension) NSF BTT-EAGER: Clean genome editing through the use of non-integrating T-DNA technology.

Gelvin, S.B. (PI), Ma, J. (co-PI), and Lee, L.-Y. (co-PI) 05/01/2020-04/29/2023 NSF TRTech-PGR: Ensifer-mediated transformation as an alternative to Agrobacterium-mediated transformation of model plants and crops

Other Activities

Editorial Board:

• Associate Editor, Plant Methods
• Associate Editor, Frontiers in Plant Science

Grant Reviews/Study Sections:

• CDFA
• Corporation for Plant Biotechnology Research
• NSF Cellular Processes

Advisory Board:

• Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan
• GENIUS, France

Faculty Presentations and Conferences, 2010-2021:

• September 9, 2021: Professor Gelvin participated in the 24th Annual NSF Plant Genome Research Program meeting, held virtually
• September 13, 2021: Professor Gelvin visited CTC Genomics Co., St. Louis, MO, where he presented a seminar entitled “Mechanism of Agrobacterium T-DNA integration into the plant genome, and generation of Agrobacterium strains that introduce T-DNA into the nucleus but do not efficiently integrate it”, and consulted with colleagues.
• September 27, 2021: Professor Gelvin visited Penn State University (virtually) by invitation from the Plant Sciences graduate students, and presented a seminar entitled “Mechanism of Agrobacterium T-DNA integration into the plant genome, and generation of Agrobacterium strains that introduce T-DNA into the nucleus but do not efficiently integrate it”. He also consulted with colleagues.
• October 11-12, 2021: Professor Gelvin, Research Scientist Dr. Lan-Ying Lee, and Drs. Chia-Ling Chang, Nana Liu, and Faozi Bukari attended (virtually) the 3rd European Agrobacterium conference.
  • Dr. Gelvin presented a talk and poster entitled “Using T-circles as a surrogate to investigate T-DNA integration into the plant genome."
  • Dr. Lee presented a talk and poster entitled “Integration-free T-DNA transfer mediated by VirD2 mutants”
  • Dr. Chang presented a poster entitled “Manipulating functional domains of DNA polymerase q to create a transgene-free genome engineering technology”.
  • Dr. Liu presented a poster entitled “ Agrobacterium ” VirE2 protein interacts with myosin VIII molecules to facilitate trafficking inside host cells.”

• October 14, 2021: Professor Gelvin presented a virtual talk to the Department of Life Sciences, Zhejiang University, Hangzhou, China entitled “Mechanism of Agrobacterium T-DNA integration into the plant genome, and generation of Agrobacterium strains that introduce T-DNA into the nucleus but do not efficiently integrate it”.
• December 5-8, 2021: Professor Gelvin presented an invited talk (virtual) at the EMBO Workshop: Plant genome stability and change 2020, entitled "No known DNA repair or recombination pathway is required for Agrobacterium T-DNA integration"

• Gelvin, S.B. 2021. Plant DNA repair and Agrobacterium T-DNA integration. Int. J. Mol. Sci. 22: 8458. doi org/10.3390/ijms22168458.
• January 24-25, 2010. "Improving Agrobacterium-mediated plant genetic transformation". Pioneer Hybrid Corp., Johnston, IO.
• March 2-5, 2010. "Agrobacterium T-DNA integration into the plant genome", Second Plant DNA Repair and Recombination Conference, Asilomar Conference Center, Pacific Grove, CA.
• July 6-8, 2010. On-site review of Department of Energy program, Oak Ridge National Laboratories, Oak Ridge, TN.
• October 1-2, 2010. "Thirty-two years of Agrobacterium research, from the plant's perspective". Retirement symposium for Dr. Eugene W. Nester, Seattle, WA.
• October 5-7, 2010. "Plant genes involved in Agrobacterium-mediated plant genetic transformation". Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, London, Ontario, Canada.
• November 5-7, 2010. The following talks and posters were presented at the 31st Annual Crown Gall Conference, University of California, Berkeley: “Direct screening of a plant cDNA library against a bait protein in planta : An application of bimolecular fluorescence complementation” by Lan-Ying Lee, Fu-Hui Wu, Chen-Tran Hsu, Shu-Chen Shen, Hsuan-Yu Yeh, De-Chih Liao, Mei-Jane Fang, Nien-Tze Liu, Choun-Sea Li, and Stanton B. Gelvin; “An Arabidopsis Myb Transcription Factor Regulates Susceptibility to Agrobacterium -mediated transformation” by Nagesh Sardesai, Huabang Chen, HoChul Yi, Gayla Olbricht, Kristin Laluk, Lin-Yun Kuang, Min-Long Wang, Yue-Ie Hsing, R.W. Doerge, Tesfaye Mengiste, and Stanton B. Gelvin.; “Involvement of Non-homologous End-joining Proteins in T-DNA Integration” by So-Yon Park, Zarir Vaghchhipawala, Balaji Vasudevan, Wanda M. Waterworth, Christopher E. West, Kiran S. Mysore, and Stanton B. Gelvin; “Arabidopsis GALLS-interacting protein (GIP) facilitates Agrobacterium -mediated transformatio by Yaling Wang, Lan-Ying Lee, Larry Hodges, Walt Ream, and Stanton B. Gelvin; “Myosins contribute to Agrobacterium ?mediated transformation” by Yanjun Yu, Lan-Ying Lee, Valerian Dolja, and Stanton B. Gelvin; “Delivery of T-DNA to DNA double stranded breaks: Mechanism and application” by Masaki Endo, Stanton B. Gelvin, and Seiichi Toki.
• December 7, 2010. “Bimolecular fluorescence complementation to image protein-protein interactions in plants.” Bimolecular Fluorescence Complementation Workshop, Purdue University, West Lafayette, IN.
• January 15-17, 2011. "Manipulating the plant genome to enhance Agrobacterium-mediated transformation." Invited speaker at the Advancement in Crop Transformation Technology Workshop, 19th Annual Plant and Animal Genome meeting, San Diego, CA.
• February 19-22, 2011. "Manipulating the plant to enhance Agrobacterium-mediated transformation." Invited speaker at the Plant Transformation Technology II Meeting, Vienna, Austria. Also, member of the International Organizing Committee.
• February 23-26, 2011. "Direct screening of a plant cDNA library against a bait protein in planta: A novel plant two-hybrid system." Invited speaker at the Plant Gene Identification Technologies Meeting, Vienna, Austria. Also, member of the International Organizing Committee.
• April 13, 2011. “Improving Agrobacterium -mediated plant genetic transformation.” Invited speaker at Dow AgroSciences, Zionsville, IN. Also, consulted with colleagues.
• May 24-27, 2011. "Manipulating the plant genome to increase susceptibility to Agrobacterium-mediated genetic transformation". Invited speaker at the Plants for the Future Symposium, University of Missouri, Columbia, MO.
• June 8, 2011. “Manipulating the plant genome to increase susceptibility to Agrobacterium -mediated transformation”. Invited speaker at the Mini-symposium on Recent Advances in Plant and Microbial Biology, Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
• June 9-10, 2011. Participated in Scientific Advisory Board meeting for the Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan. Consulted with colleagues at the IPMB during the period of June 6-June 20, 2011.
• June 17, 2011. Spoke with graduate students and consulted with colleagues at the Department of Biology, National Central University, Taoyan, Taiwan.
• July 26-28, 2011. Attended the NSF EPSCOR P3 conference in Heber Springs, Arkansas, and presented an invited talk "Manipulating the plant genome to increase susceptibility to Agrobacterium-mediated transformation".
• October 29-30, 2011. The following talks and posters were presented at the 32nd Annual Crown Gall Conference, Monsanto/Agracetus, Madison, WI: "'Classical' non-homologous end-joining proteins limit T-DNA integration into the plant genome", by So-Yon Park, Zarir E. Vaghchhipawala, Balaji Vasudevan, Wanda M. Waterworth, Christopher E. West, Stanton B. Gelvin, and Kiran S. Mysore; "Detection of VirD2 at plant double-strand DNA break sites", by Masaki Endo, Seiichi Toki, and Stanton B. Gelvin; "Plant transformation by Agrobacterium is negatively regulated by a myb transcription factor, MTF, via the cytokinin-signaling pathway", by Nagesh Sardesai, Chai-Ping Huang, and Stanton B. Gelvin; "Manipulation of myb transcription factors to improve crop transformation", by Nagesh Sardesai, Lin-Yun Kuang, Min-Long Wang, Yue-ie Hsing, Penny Sparrow, and Stanton B. Gelvin; "Root transformation assay screening of various Arabidopsis mutants", by So-Yon Park, Stanton B. Gelvin, and Zhanyuan Zhang; "RepB C-terminus mutations in ori pRi binary vectors affect plasmid copy number in Agrobacterium and transgene quality in plant transformation", by Zarir Vaghchhipawala, Xudong Ye, Sharon Radke, Mary L. Russell, Susan Johnson, Stanton B. Gelvin, and Larry Gilbertson; "Generation and screening of peptide aptamer libraries to identify inhibitors of proteins involved in Agrobacterium-mediated transformaton", by Hidekazu Iwakawa, So-Yon Park, Lan-Ying Lee, and Stanton B. Gelvin.
• November 4-6, 2011. Purdue University Biology Department retreat, Swan Lake resort, Plymouth, IN. Presented an invited talk "Manipulating the plant genome to increase susceptibility to Agrobacterium-mediated transformation".
• May 14, 2012. “Manipulating the Plant Genome to Increase Susceptibility to Agrobacterium -mediated Transformation”. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
• May 15, 2012. “Manipulating the Plant Genome to Increase Susceptibility to Agrobacterium -mediated Transformation”. Institute of Botany, Chinese Academy of Sciences, Beijing, China.
• May 19, 2012. “Manipulating the Plant Genome to Increase Susceptibility to Agrobacterium -mediated Transformation”. Keynote speaker, BIT Second Annual World Congress of Molecular & Cell Biology, Beijing, China.
• May 25, 2012. “Manipulating the Plant Genome to Increase Susceptibility to Agrobacterium -mediated Transformation”. College of Life Sciences, Zhejiang University, Hangzhou, China.
• June 3-6, 2012. Society for In Vitro Biology meeting, Seattle, WA. “Increasing Agrobacterium -mediated Genetic Transformation by Manipulating the Plant Genome”.
• June 20-July 16, 2012. Visited the Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan. Delivered a series of lectures on molecular biology techniques to graduate students, and discussed research with collaborators.
• August 31, 2012. “Manipulating the plant genome to increase susceptibility to Agrobacterium -mediated transformation”. Department of Biology, IUPUI.
• November 7, 2012. "Manipulating the plant genome to increase susceptibility to Agrobacterium -mediated transformation”. Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., 612 65 Brno, and Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic. Consulted with colleagues Nov. 5-7.
• November 9, 2012. "Manipulating the plant genome to increase susceptibility to Agrobacterium -mediated transformation”. School of Medicine, University of Montpellier, Nimes, France.
• December 1-2, 2012. The following oral presentations and posters were made by the Gelvin laboratory at the 33 ^rd annual Crown Gall Conference at Hiram College, Hiram, Ohio: Nagesh Sardesai, Lan-Ying Lee, Huabang Chen, HoChul Yi, Gayla R. Olbricht, Alexandra Stirnberg, Jacob Jeffries, R.W. Doerge, and Stanton B. Gelvin. “ Agrobacterium -mediated transformation is negatively regulated by a Myb transcription factor via the cytokinin-signaling pathway and a membrane protein important for bacterial attachment: Application to crop transformation”. Lan-Ying Lee, Yong Shi, Yanjun Yu, Shengjie Xu, Valerian Dolja, and Stanton B. Gelvin. “Myosins affect the subcellular localization of Agrobacterium Virulence effector proteins”. Hidekazu Iwakawa and Stanton B. Gelvin. “Role of Histone H3 lysine 27 in Agrobacterium -mediated transformation”. Zarir Vaghchhipawala, So-Yon Park, Balaji Vasudevan, Wanda M. Waterworth, Zhanyuan Zhang, Christopher E. West, Stanton B. Gelvin, and Kirankumar S. Mysore. “ Agrobacterium may delay plant non-homologous end-joining DNA repair to favor T-DNA integration”.
• December 9-11, 2012. “Manipulating the plant genome to increase susceptibility to Agrobacterium -mediated transformation” and “Proposed research collaborations”. Bayer Crop Science LP, Research Triangle Park, NC.
• February 1, 2013. “Manipulating the plant genome to increase susceptibility to Agrobacterium -mediated transformation” . Department of Molecular Genetics, Ohio State University, Columbus, OH. And, consulted with colleagues.
• March 4, 2013. "Enhancing Agrobacterium-mediated genetic transformation by manipulating the host genome". NIH, Bethesda, MD.
• March 5, 2013. "A plant global regulator of Agrobacterium-mediated transformation: Application to crop transformation". Corporation for Plant Biotechnology Research (CPBR) Symposium. Washington, DC.
• March 21-April 2, 2013. Visited the Institute for Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan. Conducted research and consulted with colleagues on a joint research grant.
• March 29, 2013. Presented a seminar “Manipulating the plant genome to increase susceptibility to Agrobacterium -mediated transformation” at the Department of Bioagricultural Science, National Chiayi University, Chiayi, Taiwan.
• May 19-30, 2013. Participated on the search committee for a new director for the Institute for Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, and consulted with colleagues.
• July 23-August 7, 2013. Conducted research and taught a graduate course at the Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
• July 29, 2013. “Manipulating the plant genome to increase susceptibility to Agrobacterium -mediated transformation”. Department of Life Sciences, National Tsing-Hua University, Hsinchu, Taiwan.
• October 7-17, 2013. “Manipulating the plant genome to increase susceptibility to Agrobacterium -mediated transformation”. Max Planck Institute for Plant Breeding Research, Cologne, Germany; University of Basel, Basel, Switzerland; University of Wuerzburg, Wuerzburg, Germany.
• November 3-5, 2013. “Manipulating the plant genome to increase susceptibility to Agrobacterium -mediated transformation”. Interdisciplinary Plant Group, University of Missouri.
• November 19-21, 2013. Co-organized and participated in an Agrobacterium T-DNA integration workshop at the Noble Foundation, Ardmore, OK.
• November 22-24, 2013. Stanton Gelvin, Fang-Yu Hsu, Rachelle Lapham, Lan-Ying Lee, Yong Shi, and Kamy Singer attended the 34 ^th Annual Crown Gall Conference at DuPont-Pioneer, Johnston, IA. The following presentations were made both orally and as posters:
  • So-Yon Park, Zarir Vaghchhipawala, Balaji Vasudevan, Lan-Ying Lee, Kamy
    Singer, Wanda M. Waterworth, Zhanyuan J. Zhang, Christopher E. West, Stanton B. Gelvin, and Kirankumar S. Mysore. “Non-Homologous End-Joining Proteins Limit Agrobacterium T-DNA Integration Into the Plant Genome.”
  • Kamy Singer, Lan-Ying Lee, Jiangming Li, Tzvi Tzfira, and Stanton B. Gelvin. “Do plant T-circles reflect T-DNA integration events?”
  • Yong Shi, Lan-Ying Lee, Stanton B. Gelvin. “Is VIP1 important for Agrobacterium -mediated transformation?”
  • Wei Wei, Yaling Wang, Lan-Ying Lee, Larry D. Hodges, Walt Ream, and Stanton B. Gelvin. “Transcriptome analysis of Arabidopsis thaliana roots overexpressing GIP, a putative transcription factor important for Agrobacterium -mediated plant transformation.”
  • So-Yon Park, Stanton B. Gelvin, and Zhanyuan Zhang. “Heat shock protein 90.1 interacts with VIP1 and plays a role in Agrobacterium -mediated plant transformation.”

• January 28, 2014. “Manipulating the Plant Genome to Increase Susceptibility to Agrobacterium -mediated Transformation”. Department of Botany, Purdue University.
• February 11, 2014. “Manipulating the Plant Genome to Increase Susceptibility to Agrobacterium -mediated Transformation”. Gregor Mendel Institute, Vienna, Austria.
• February 12-14, 2014. Attended the Plant Transformation Technologies III conference in Vienna, Austria. Presented an invited talk "Agrobacterium-mediated transformation is negatively regulated by a Myb transcription factor: Application to crop transformation" coauthored by Nagesh Sardesai, Lan-Ying Lee, Huabang Chen, HoChul Yi, Gayla R. Olbricht, Alexandra Stirnberg, Jacob Jeffries, and R.W. Doerge. Dr. Lan-Ying Lee presented an invited talk "Peptide aptamers to generate novel plant phenotypes and investigate protein-peptide interactions" coauthored by Yanjun Yu, Joerg Spantzel, Yaling Wang, Soyon Park, Hidekawa Iwakawa, and Stanton B. Gelvin.
• March 16-24, 2014. Visited the Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan. Worked with research group on a funded project and consulted with colleagues.
• June 9-14, 2014. Visited the Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan. As a member of the Scientific Advisory Board for the IPMB, participated in the SAB review of the Institute. Presented a seminar at a Mini-Symposium entitled "New insights on the mecanism of Agrobacterium T-DNA integration into the plant genome". Also, reviewed research progress on a project jointly conducted with IPMB members.
• August 4-8, 2014. Taught a mini-course and conducted research at the Institute for Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
• August 10-15, 2014. Attended the International Association of Plant Biotechnology Congress in Melbourne, Australia. Presented a Keynote talk entitled: "A plant myb transcription factor negatively regulates Agrobacterium-mediated transformation: Application to crop transformation".
• October 14-16, 2014. Organized at Purdue University the 35th Annual Crown Gall Conference. The following talks were presented, along with posters:

Fang-Yu Hsu, Yong Shi, Lan-Ying Lee, Yanjun Yu, Shengjie Xu, Valerian Dolja, and Stanton B. Gelvin. " A rabidopsis myosins affect the subcellular pattern of Agrobacterium virulence proteins".

Stanton B. Gelvin, So-Yon Park, Zarir Vaghchhipawala, Balaji Vasudevan, Lan-Ying Lee, Yunjia Shen, Kamy Singer, Wanda M. Waterworth, Zhanyuan J. Xhang, Christopher E. West, and Kirankumar S. Mysore. "Known non-homologous ending joining pathways are not required for T-DNA integration."

Fu-Jin Wei, Lin-Yun Kuang, Lan-Ying Lee, Stanton Gelvin, and Yue-Ie Hsing. "Inspecting genome changes among regenerants and transformants via NGS data."

The following posters were presented:

Kamy Singer, Laurel Jahn, Lan-Ying Lee, and Stanton B. Gelvin. "Do plant T-circles reflect T-DNA integration events?"

Rachelle Lapham, Lan-Ying Lee, and Stanton B. Gelvin. "Potential novel role of the Agrobacterium virulence effector protein VirE2 in modulating plant gene expression."

Wei Wei, Yaling Wang, Lan-Ying Lee, Maciei Maselko, Larry D. Hodges, Walt Ream, and Stanton B. Gelvin. " Agrobacterium rhizogenes effector protein GALLS interacts with host transcription factor LSH10 and stimulates Agrobacterium -mediated transformation."

• November 23-27, 2014. Dr. Gelvin attended the International Scientific Advisory Committee meeting for GENIUS (Genome ENgineering Improvement for Useful plants of a Sustainable agriculture) in Paris, France.
• March 3-4, 2015. Dr. Gelvin attended the annual CPBR meeting in Washington, DC where he presented a poster entitled "Regulation of T-DNA integration into plant genomes"
• March 15-22, 2015. Dr. Gelvin visited the Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan where he consulted with colleagues and presented a seminar entitled "New insights on the mechanism of Agrobacterium T-DNA into the plant genome". He also presented this seminar on March 16, 2015 at National Taiwan University.
• August 2-7, 2015. Dr. Stanton Gelvin visited the Shanghai Centre for Plant Stress Biology. He presented a seminar “New insights on the mechanism of Agrobacterium T-DNA integration into the plant genome”. He consulted with colleagues, and organized research collaborations between his laboratory at Purdue University and several laboratories at the Centre.
• August 8-21, 2015. Dr. Stanton Gelvin visited the Institute of Plant and Microbial Biology at the Academia Sinica, Taipei Taiwan. He taught a course in the Taiwan International Graduate Program, consulted with colleagues, and worked with his collaborative research group.
• August 16-17, 2015. Dr. Stanton Gelvin visited National Cheng Kung University, Tainan, Taiwan, where he presented a seminar “New insights on the mechanism of Agrobacterium T-DNA integration into the plant genome” and consulted with colleagues.
• September 14-15, 2015. Dr. Gelvin attended the 3 ^rd Plant Genomics Congress USA in St. Louis, MO, where he presented a talk entitled “RNAseq analysis reveals Arabidopsis genes important for Agrobacterium -mediated plant genetic transformation”.
• November 14-15, 2015. Stanton Gelvin, Fang-Yu Hsu, Rachelle Lapham, and Lan-Ying Lee attended the 36 ^th annual Crown Gall Conference at DowAgrosciences, Zionsville, IN. The following posters and oral presentations were made:
  1. Fang-Yu Hsu, Lan-Ying Lee, Yong Shi, Yanjun Yu, Shengjie Xu, Praveen Rao, Valerian Dolja, and Stanton B. Gelvin: The Arabidopsis actin cytoskeleton affects the subcellular distribution of the Agrobacterium effector protein VirE2.
  2. Rachelle Lapham, Lan-Ying Lee, and Stanton B. Gelvin: Potential novel role of the Agrobacterium virulence effector protein VirE2 in modulating plant gene expression.
  3. Stanton B. Gelvin, Yunjia Shen, Emily Kim, Kamy Singer, Laurel Jahn, Lan-Ying Lee, Amita Kaundal, and Kirankumar S. Mysore: How important are plant DNA repair and recombination pathways for T-DNA integration?

• November 15-17, 2015. Stanton Gelvin attended a NSF-sponsored workshop on plant transformation at Clearwater Beach, Florida.
• July 17-21, 2016. Stanton Gelvin presented a talk at the 2016 international IS-MPMI (International Society for Molecular Plant-Microbe Interactions) conference at a special session on “Recent advances Agrobacterium biology” entitled “Potential novel role for the Agrobacterium virulence effector protein VirE2 in modulating plant gene expression”. Work from his laboratory was also featured in a presentation by Kiran S. Mysore entitled “Activity of plant non-homologous end-joining DNA repair proteins is not required for Agrobacterium T-DNA integration”.
• August 15-16, 2016. Stanton Gelvin presented the Keynote Address at the 33 ^rd Annual Mid-Atlantic Plant Molecular Biology Society meeting at Patuxent National Wildlife Center, Maryland. The talk was entitled “Plant genes important for Agrobacterium -mediated genetic transformation”.
• September 9-20, 2016. Stanton Gelvin attended the first Euro-Mediterranean Agrobacterium Meeting in Gif-sur-Yvette, France. He chaired a session, and presented an invited talk entitled "New insights into the mechanism of Agrobacterium T-DNA integration into the plant genome." Other members of the Gelvin laboratory also presented invited talks: Lan-Ying Lee "VIP1 and its family members are not important for Agrobacterium-mediated transformation", and Rachelle Lapham "Potential novel role for the Agrobacterium virulence effector protein VirE2 in modulating plant gene expression". Drs. Gelvin and Lee also visited colleagues in Strasboug, France and Basel, Switzerland.
• October 17-19, 2016. Stanton Gelvin and Lan-Ying Lee attended the 2016 International Symposium on Plant Transformation Biotechnologies at the Academia Sinica, Taipei, Taiwan. Dr. Gelvin presented a talk entitled "Higher order Arabidopsis DNA repair and recombination mutants integrate increasing amounts of Agrobacterium T-DNA into the plant genome." Dr. Lee presented a talk entitled "VIP1 and its family members are not important for Agrobacterium-mediated transformation." Drs. Gelvin and Lee also consulted with colleagues at the Academia Sinica.
• November 4-6, 2016. Professor Gelvin and laboratory members Dr. Lan-Ying Lee, Rachelle Lapham, and Ashok Govindan attended the 37 ^th Annual Crown Gall Conference at Indiana University, Bloomington, IN. The following talks and posters were presented:
  • Stanton B. Gelvin, Soyon Park, Yunjia Shen, Emily Kim, Lan-Ying Lee, Gabriela Tenea, Heiko Oltmanns, Amita Kaundal, and Kirankumar S. Mysore: “Known plant DNA repair and recombination pathways are not mportant for T-DNA integration.”
  • Lan-Ying Lee, Rachelle Lapham, Yong Shi, Sanghun Lee, Tesfaye Mengiste, Daisuke Tsugama, and Stanton B. Gelvin: “VIP1 and its family members are not important for Agrobacterium -mediated transformation.”
  • Rachelle Lapham, Lan-Ying Le, and Stanton Gelvin: “Potential novel role of the Agrobacterium virulence effector protein VirE2 in modulating plant gene expression.”
  • Ashok Govindan also presented a poster: Ashokkumar Govindan, Fiona Doohan, Stanton B. Gelvin, and Ewen Mullins: “Understanding the plant response to Ensifer -mediated transformation (EMT): Identifying potential regulators of EMT by Arabidopsis transcriptomic and T-DNA mutant analyses.”
• November 23-25, 2016. Stanton Gelvin participated in a Scientific Advisory Board meeting for the GENIUS project in Paris, France. He also attended a plant CRISPR symposium.
• February 23-April 23, 2017. Worked in the laboratory of Dr. Seiichi Toki, National Institute of Agrobiological Sciences, Tsukuba, Japan.
• March 2-3, 2017. Presented a seminar at Chubu University, Nagoya, Japan entitled “New insights on the mechanism of Agrobacterium T-DNA integration into the plant genome” and consulted with colleagues at Chubu University and Nagoya University, Nagoya, Japan.
• March 10, 2017. Presented a seminar at the National Agricultural Research Organization, Tsukuba, Japan entitled “New insights on the mechanism of Agrobacterium T-DNA integration into the plant genome”.
• April 5, 2017. Presented a seminar at RIKEN, Yokohama, Japan entitled “Plant genes important for Agrobacterium -mediated plant transformation”, and consulted with colleagues.
• April 6, 2017. Consulted with colleagues at the University of Tokyo.
• April 13, 2017. Presented a seminar at Japan Tobacco Co., Iwata, Japan entitled “New insights on the mechanism of Agrobacterium T-DNA integration into the plant genome”.
• April 17-18, 2017. Consulted with Dr. Daisuke Tsugama from Hokaido University, Japan.
• May 30-June 3, 2017. Served on the advisory board for SweTree Technologies Company at Umea, Sweden.
• June 27-July 5, 2017. Attended the VISCEA conferences on Plant Transformation and Biotechnology IV, and Plant Genome Editing and Genome Engineering in Vienna, Austria. Served on the International Organizing Committee for the Plant Transformation and Biotechnology IV conference. Served as a session chair and presented a major talk at the Plant Transformation and Biotechnology IV conference entitled "Known plant DNA repair and recombination pathways are not important for T-DNA integration".
• September 7-8, 2017. Professor Gelvin attended the 20 ^th Annual NSF Plant Genome Program meeting for Principal Investigators in Arlington, VA . He presented a poster entitled “Manipulating Agrobacterium -mediated Transformation and T-DNA Integration for Plant Synthetic Biology and Genome Engineering.”
• October 6-8, 2017. Professor Gelvin, Research Scientist Dr. Lan-Ying Lee, Graduate student Rachelle Lapham, and Visiting scientist Hiroaki Saika attended the 38 ^th Crown Gall conference at Oregon State University, Corvallis, Oregon. The following talks and posters were presented
• September 13, 2021. Professor Gelvin visited CTC Genomics Co., St. Louis, MO, where he presented a seminar entitled “Mechanism of Agrobacterium T-DNA integration into the plant genome, and generation of Agrobacterium strains that introduce T-DNA into the nucleus but do not efficiently integrate it”, and consulted with colleagues.
• September 27, 2021. Professor Gelvin visited Penn State University (virtually) by invitation from the Plant Sciences graduate students, and presented a seminar entitled “Mechanism of Agrobacterium T-DNA integration into the plant genome, and generation of Agrobacterium strains that introduce T-DNA into the nucleus but do not efficiently integrate it”. He also consulted with colleagues.
• October 11-12, 2021. Professor Gelvin, Research Scientist Dr. Lan-Ying Lee, and Drs. Chia-Ling Chang, Nana Liu, and Faozi Bukari attended (remotely) the 3 ^rd European Agrobacterium conference.
  • Dr. Gelvin presented a talk and poster entitled “Using T-circles as a surrogate to investigate T-DNA integration into the plant genome”.
  • Dr. Lee presented a talk and poster entitled “Integration-free T-DNA transfer mediated by VirD2 mutants”
  • Dr. Chang presented a poster entitled “Manipulating functional domains of DNA polymerase q to create a transgene-free genome engineering technology”.
  • Dr. Liu presented a poster entitled “Agrobacterium” VirE2 protein interacts with myosin VIII molecules to facilitate trafficking inside host cells.”

Stanton B. Gelvin, Ayako Nishizawa-Yokoi, Hiroaki Saika, Naho Hara, Lan-Ying Lee, and Seiichi Toki: “Rice and Arabidopsis DNA polymerase θ ( polQ ) mutants can integrate T-DNA”

Lan-Ying Lee, Fang-Yu Hsu, Eder Xhako, and Stanton B. Gelvin: “Nuclear localization of VirE2 abolishes its function in transformation”

Rachelle Lapham and Stanton B. Gelvin: “Role of the Agrobacterium virulence effector protein VirE2 in modulating plant gene expression”

Hiroaki Saika, Akiko Mori, and Seiichi Toki: “Toward establishing a universal site-directed mutagenesis system via gene targeting using Agrobacterium in rice”

• November 19-24, 2017. Professor Gelvin participated in an international scientific review of projects conducted at the National Institute of Agrobiological Sciences, National Agriculture and Forestry Organization, Tsukuba, Japan. He presented seminars entitled “Plant genes important for Agrobacterium -mediated transformation” at a symposium in Tsukuba, and at Hokkaido University, Sapporo, Japan. He also consulted with colleagues both in Tsukuba and Sapporo.

• January 28-March 30, 2018. Professor Gelvin and Research Scientist Dr. Lan-Ying Lee worked in the laboratory of Dr. Seiichi Toki, National Agricultural Research Organization, National Institute of Agrobiological Sciences, Tsukuba, Japan

• June 4, 2018. Dr. Gelvin co-chaired the session “Systems to deliver genome engineering reagents to plant cells” at the Society for In Vitro Biology (SIVB) meeting in St. Louis, Missouri. He also presented an invited lecture “Improving Agrobacterium -mediated delivery of genome editing reagents: Potential for improving T-DNA delivery and regulating T-DNA integration”.
• June 13-14, 2018. Professor Gelvin and Research Scientist Dr. Lan-Ying Lee participated in an Advisory Committee meeting for SweTree Technologies, Umea, Sweden.

• August 19-23, 2018: Professor Stanton Gelvin and Research Scientist Dr. Lan-Ying Lee attended the International Association of Plant Biotechnology (IAPB) congress in Dublin, Ireland. Dr. Gelvin presented a Keynote talk entitled “Mutation of a Plant Myb Transcription Factor Increases Susceptibility to Agrobacterium -mediated Transformation”. Dr. Lee presented a poster entitled “Generation of Agrobacterium Strains that Efficiently Introduce but Do Not Integrate T-DNA into the Plant Genome”. This poster won an award for best poster of the meeting.

• September 12-13, 2018: Prof. Stanton Gelvin, Research Scientist Dr. Lan-Ying Lee, and graduate student Rachelle Lapham attended the Agrobacterium 2018 conference at Ghent, Belgium. Prof. Gelvin presented a talk entitled “No known DNA repair or recombination pathway is required for Agrobacterium T-DNA integration” and a poster entitled “Rice and Arabidopsis DNA polymerase θ ( polQ ) mutants can integrate T-DNA”. Dr. Lee presented a talk and poster entitled “Directing VirE2 to the host nucleus abolishes its function in transformation”. Rachelle Lapham presented a talk and poster entitled “Role of the Agrobacterium virulence effector protein VirE2 in modulating plant gene expression”.

• December 15-23, 2018: Prof. Stanton Gelvin visited the Rubber Tree Research Institute, Chinese Academy of Tropical Agricultural Sciences, Heikou, Hainan, China where he presented three seminars entitled "Plant genetic engineering using Agrobacterium -mediated transformation"; "A plant myb transcription factor negatively regulates Agrobacterium-mediated transformation: Application to crop transformation"; and "Mechanism and control of T-DNA integration into the plant genome". He also consulted with colleagues.

  March 1-25, 2019. Professor Stanton Gelvin and Research Scientist Dr. Lan-Ying Lee visited multiple institutions in Taiwan and China and participated in the following activities: National Cheng Kung University, Tainan, Taiwan: Taught a micro-course (lectures and laboratories) on Agrobacterium biology and protein-protein interactions in plants (Gelvin and Lee)

• Seminar, National Cheng Kung University, Tainan, Taiwan: “Mechanism and control of T-DNA integration into the plant genome” (Gelvin)

• Seminar, Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan: “Mechanism and control of T-DNA integration into the plant genome” (Gelvin)

• Seminar, Zhejian University, Hangzhou, China: “Mechanism and control of T-DNA integration into the plant genome” (Gelvin)

• Seminar, Shanghai Institute of Plant Physiology and Ecology, Shanghai, China: “Mechanism and control of T-DNA integration into the plant genome” (Gelvin)

• Seminar, Shanghai Center for Plant Stress Biology, Shanghai, China: “Mechanism and control of T-DNA integration into the plant genome” (Gelvin and Lee)

• April 26, 2019. Professor Stanton Gelvin presented a seminar at the Fifth Midwest Membrane Trafficking and Signaling Symposium, Purdue University, entitled “A plant myb transcription factor negatively regulates Agrobacterium -mediated transformation”.

• May 6-7, 2019. Professor Gelvin visited Inari Agriculture Corp. where he presented two seminars entitled “Manipulating Agrobacterium T-DNA integration” and “ Ensifer as an alternative to Agrobacterium for plant genetic transformation”, and consulted with colleagues.

• June 18-21, 2019. Professor Stanton Gelvin and Research Scientist Dr. Lan-Ying Lee visited several institutions in St. Louis, MO:

  • June 18: At KWS Gateway Research Center, LLC. Prof. Gelvin presented two seminars entitled “Manipulating T-DNA integration” and “ Ensifer as an alternative to Agrobacterium for plant genetic transformation”. They also consulted with colleagues.

  • June 19: Professor Gelvin and Dr. Lee met with representatives from TechAccel. Professor Gelvin and Dr. Lee met with scientists from Centro de Tecnologia Canavierira (CTC) and discussed issues of plant genome engineering.

  • June 20-22: Professor Gelvin and Dr. Lee presented a lecture and laboratory workshop for ~50 scientists at the Donald Danforth Plant Science Center on “Fundamentals and Practice of Agrobacterium- mediated plant transformation”. They were joined in their presentations by Drs. Bill Gordon-Kamm, Veena Veena, and Kan Wang.

• October 25-27, 2019. Professor Stanton B. Gelvin, Research Scientist Dr. Lan-Ying Lee, post-doctoral research fellow Vinay Kumar, former graduate student Rachelle Lapham, visiting graduate student Nivya V.M., and visiting scientist Dr. Jasmine Shah attended the 40 ^th Annual Crown Gall conference at the University of Missouri, Columbia, MO. The following talks and posters were presented

  • Stanton B. Gelvin (with Ayako Nishizawa-Yokoi, Hiroaki Saika, Lan-Ying Lee, Yunjia Shen, Yooyoung Kim, and Seiichi Toki): “No known DNA repair or recombination pathway is required for Agrobacterium T-DNA integration”.

  • Rachelle Lapham (with Lan-Ying Lee, Eder Xhako, Esteban Ganan Gomez, and Stanton B. Gelvin): “ Agrobacterium VirE2 protein modulates plant gene and protein expression to facilitate transformation from its location in the plant cytoplasm”.

• Lan-Ying Lee (with Kirankumar S. Mysore and Stanton B. Gelvin): Generation of Agrobacterium strains that introduce T-DNA into the nucleus but do not efficiently integrate it into the plant genome”.

  Stanton B. Gelvin (with Rachelle Lapham, Ashok Govindan, Caleigh Roleck, and Ewen Mullins): “A reduced host defense response supports Ensifer -mediated transformation as an alternative to Agrobacterium -mediated transformation”.
• December 11, 2019-February 14, 2020. Professor Gelvin and Research Scientist Dr. Lan-Ying Lee visited the laboratory of Dr. Seiichi Toki, NARO, Tsukuba, Japan where they conducted research on the mechanism Agrobacterium T-DNA integration into the rice genome.

• June 19: Professor Gelvin and Dr. Lee met with representatives from TechAccel. Professor Gelvin and Dr. Lee met with scientists from Centro de Tecnologia Canavierira (CTC) and discussed issues of plant genome engineering.

• June 20-22, 2019: Professor Gelvin and Dr. Lee presented a lecture and laboratory workshop for ~50 scientists at the Donald Danforth Plant Science Center on “Fundamentals and Practice of Agrobacterium- mediated plant transformation”. They were joined in their presentations by Drs. Bill Gordon-Kamm, Veena Veena, and Kan Wang.

• March 23, 2020. Professor Gelvin presented a seminar at Penn State University entitled “Mechanisms and control of Agrobacterium T-DNA integration into the plant genome.”

• February 3, 2020. Professor Gelvin presented a seminar at NARO, Tsukuba, Japan entitled “Characterization of T-DNA/plant DNA junctions from rice NHEJ mutants”

• January 20, 2020. Professor Gelvin and Research Scientist Lan-Ying Lee visited Kyushu Technological University, Japan, where Professor Gelvin presented a seminar entitled “ Agrobacterium T-DNA integration into the plant genome“

• January 15, 2020. Professor Gelvin and Research Scientist Lan-Ying Lee presented seminars at NARO, Tsukuba, Japan entitled “A reduced host defense response supports Ensifer -mediated transformation as an alternative to Agrobacterium­ -mediated transformation” and “Generation of Agrobacterium strains that introduce T-DNA into the nucleus but do not efficiently integrate it into the plant genome”, respectively.
• April 28, 2021: Professor Gelvin presented the first lecture in the webinar series “A closer look: Learning from the past—Considering the future” presented by the Society for In Vitro Biology (SIVB). His seminar was titled “Plant genes important for T-DNA trafficking and integration in plant cells”.
• May 27, 2021: Professor Gelvin presented two virtual seminars to Pairwise Plants, Durham, North Carolina, entitled “Generation of Agrobacterium strains that introduce T-DNA into the nucleus but do not efficiently integrate it into the plant genome” and “Developing Ensifer -mediated plant transformation as an alternative to Agrobacterium -mediated transformation”.
• June 5-9, 2021: Professor Gelvin attended the Society for In Vitro Biology (SIVB) virtual meeting. He participated in the SIVB Biotechnology Program Committee meeting, and presented a seminar entitled “Developing Ensifer -mediated plant transformation as an alternative to Agrobacterium -mediated transformation”.